目的 对中药冬虫夏草进行鉴别研究, 建立其传统生药学与现代DNA分子鉴别方法。方法 采用性状、显微鉴别方法与RAPD-SCAR标记对中药冬虫夏草进行鉴别研究。结果 在性状鉴别的基础上, 详细描述了中药冬虫夏草的横切面结构与粉末特征, 提供了直观、真实的药材性状及显微鉴别数码成像图谱;将冬虫夏草特异性的RAPD扩增片段转化为SCAR标记, 在优化的鉴别PCR条件下可以将冬虫夏草鉴别。结论 性状、显微鉴别方法形象直观, 而DNA分子标记鉴别方法简单易行, 为冬虫夏草药材真伪鉴别提供了新的科学依据。
Abstract
OBJECTIVE To study on the identification of Cordyceps and establish its traditional pharmacognostic identification and modern identification method based on DNA molecular marker technology. METHODS The METHODS of macroscopic, microscopic examination and RAPD-SCAR were employed here to authenticate Cordyceps. RESULTSBased on the macroscopic identification, the features of transverse section and powder of Cordyceps were described in detail. The digital photographs were presented here in revealing the main macroscopic and microscopic characteristic of Cordyceps. The specific RAPD fragment of Cordyceps was conversed into SCAR marker, and Cordyceps could be identified by the optimized PCR conditions. CONCLUSION The authenticated method of microscopic and macroscopic identification is intuitive. However, the identification method based on DNA molecular marker is sample, which provides the new scientific evidences for the identification of authenticity of Cordyceps.
关键词
冬虫夏草 /
生药学 /
DNA分子标记 /
鉴别
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Key words
Cordyceps /
pharmacognosy /
DNA molecular marker /
identification
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中图分类号:
R284
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参考文献
Ch.P(2010)Vol I(中国药典2010年版一部). 2010:412. FENG K, WANG S, HU D J, et al. Random amplified polymorphic DNA (RAPD) analysis and the nucleosides assessment of fungal strains isolated from natural Cordyceps sinensis . J Pharm Biomed Anal, 2009, 50(3): 522-526. ZHANG Z Z, WANG J C, CHU Z Y, et al. Identification analysis of Cordyceps sinencsis and its common adulterant . Chin J Med Guide(中国医药导刊), 2009, 7(11): 1244, 1255. DUAN J H, YANG Y Q. Identification of Cordyceps sinencsis and its adulterant . World J Integ Trad and West Med (世界中西医结合杂志), 2011, 6(12): 1037-1041. GAO M, WANF J S, ZENG J L, et al. Identification of Cordyceps sinesnsis and its counterfeits. J Chin Med Mater (中药材). 2011, 34(2): 213-216. KANG S, HUO H M, ZHENG J, et al. Plarmacognostic authentication study of Cordyceps and its uncertified product-Daishichongcao . China J Pharm Anal(药物分析杂志), 2011, 31(6): 1035-1039. CHEN S T, LIU B L, ZHAO Z Z, et al. Studies on macroscopic and microscopic identification of Cordyceps sinesnsis and its counterfeits . China J Chin Mater Med (中国中药杂志), 2011, 9(36): 1141-1144.
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脚注
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